human ifgf23 (Quidel)
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human ifgf23
Human Ifgf23, supplied by Quidel, used in various techniques. Bioz Stars score: 95/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+ifgf23/FGF-23+ELISA/pmc12032739-69-68-73
Average 95 stars, based on 74 article reviews
Human Ifgf23, supplied by Quidel, used in various techniques. Bioz Stars score: 95/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+ifgf23/FGF-23+ELISA/pmc12032739-69-68-73
Average 95 stars, based on 74 article reviews
human ifgf23 - by Bioz Stars,
2026-10
95/100 stars
Images
1) Product Images from "The Roxadustat (FG-4592) ameliorates tubulointerstitial fibrosis by promoting intact FGF23 cleavage"
Article Title: The Roxadustat (FG-4592) ameliorates tubulointerstitial fibrosis by promoting intact FGF23 cleavage
Journal: Cell Communication and Signaling : CCS
doi: 10.1186/s12964-025-02175-2
Figure Legend Snippet: FG-4592 lowers iFGF23 level in CKD rats and CKD patients. Parameters measured include ( A ) Serum intact fibroblast growth factor 23 (iFGF23) levels in CKD rats. B Serum total FGF23 levels in CKD rats. C Serum iFGF23 levels in CKD patients. D Serum total FGF23 levels in CKD patients. E Scatter plots with linear regression analysis demonstrating a significant correlation between blood urea nitrogen (BUN) and iFGF23 levels in CKD rats. F Scatter plots with linear regression analysis showing a significant correlation between Scr and iFGF23 levels in CKD rats. G Scatter plots with linear regression analysis revealing a significant correlation between iFGF23 levels and the percentage of fibrotic area in the kidney of CKD rats. H Scatter plots with linear regression analysis indicating a significant correlation between iFGF23 levels and Collagen 1 staining intensity in the kidney of CKD rats. I Scatter plots with linear regression analysis illustrating a significant correlation between iFGF23 levels and Fibronectin staining intensity in the kidney of CKD rats. J Scatter plots with linear regression analysis demonstrating a significant correlation between iFGF23 levels and α-SMA staining intensity in the kidney of CKD rats. Data are presented as means ± SD ( n = 5). Statistical significance is denoted as follows: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
Techniques Used: Staining
Figure Legend Snippet: iFGF23 mediates FG-4592-induced inhibition of tubular cell fibrogenesis. HK-2 cells were pre-treated with TGF-β1 (10 ng/mL) for 24 h, followed by treatment with recombinant FGF23 (rFGF23, 50 ng/mL) or vehicle for an additional 24 h. Parameters measured include: A COL1A1 mRNA expression levels in HK-2 cells, as determined by quantitative PCR (qPCR). B TGFB1 mRNA expression levels in HK-2 cells, as determined by qPCR. C Collagen 1 protein expression levels in HK-2 cells, as assessed by Western blot analysis. D Schematic illustration of the co-culture system using UMR-106 cells and HK-2 cells in a Transwell setup. E mRNA expression levels of Fn1 , Acta2 , and Col1a1 in HK-2 cells, as quantified by qPCR. Data are presented as means ± SD ( n = 5). Statistical significance is denoted as follows: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001
Techniques Used: Inhibition, Recombinant, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Co-Culture Assay
Figure Legend Snippet: FG-4592 ameliorates TIF by inhibiting iFGF23-WNT5A pathway. Parameters measured include ( A ) Hierarchical clustering analysis revealing distinct mRNA expression profiles among the experimental groups. Yellow and blue shades indicate expression levels above and below the relative mean, respectively, across all samples. B Gene Ontology (GO) enrichment analysis. C Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. D and F mRNA and protein expression levels of Wnt5a and β-catenin in the following groups: sham, CKD + vehicle, and CKD + FG-4592. E and G mRNA and protein expression levels of Wnt5a and β-catenin in the following groups: CKD + FG-4592 + vehicle and CKD + FG-4592 + rFGF23. H and I Representative immunohistochemical images showing Wnt5a and β-catenin expression in kidney tissues across different experimental groups. J mRNA expression levels of COL1A1 , ACTA1 , WNT5A , and CTNNB1 in HK-2 cells following WNT5A siRNA interference, as determined by quantitative PCR (qPCR). K Protein expression levels of Collagen 1, Wnt5a, and β-catenin in HK-2 cells after WNT5A siRNA interference, as assessed by Western blot analysis. Scale bars: 50 μm. Data are presented as means ± SD ( n = 5). Statistical significance is denoted as follows: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
Techniques Used: Expressing, Immunohistochemical staining, Real-time Polymerase Chain Reaction, Western Blot
Figure Legend Snippet: FG-4592 lowers iFGF23 levels through Furin-mediated cleavage. Parameters measured include ( A ) mRNA expression levels of Furin , Galnt3 , and Fam20c in bone tissues from the following groups: sham, CKD + vehicle, and CKD + FG-4592, as determined by quantitative PCR (qPCR). B Protein levels of HIF-1α, Furin, and intact fibroblast growth factor 23 (iFGF23) in bone tissues treated with FG-4592 or vehicle, as assessed by Western blot analysis. C Representative images of hematoxylin and eosin (HE) staining of bone tissues (scale bars: 20 μm), along with IHC staining for HIF-1α and iFGF23 (scale bars: 50 μm) and Furin (scale bars: 100 μm). D UMR-106 cells were pretreated with 10 nM 1,25(OH) 2 D 3 , and the expression of FGF23 mRNA was analyzed using real-time PCR. E Western blot analysis was performed to measure iFGF23 protein expression in UMR-106 cells. Cells were pretreated with indoxyl sulfate (IS, 0.5 mM) or vehicle, followed by treatment with FG-4592 (50 μM) or vehicle for 24 h. F Levels of iFGF23 and total FGF23 in the cell supernatant were quantified using an ELISA assay kit. G Western blot analysis was used to evaluate the protein expression of iFGF23 and Furin in UMR-106 cells. H Motif map of the Furin promoter region, highlighting the HIF-1 binding site. I ChIP assays demonstrated HIF-1 binding to the Furin promoter, which was enhanced by FG-4592 stimulation (50 μM for 24 h). J Protein expression levels of Furin and iFGF23 in UMR-106 cells following Furin siRNA interference, as determined by Western blot analysis. K Levels of iFGF23 and total FGF23 in the cell supernatant were measured using an ELISA assay kit after Furin siRNA interference. Data are presented as means ± SD ( n = 5). Statistical significance is denoted as follows: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Staining, Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Binding Assay
Figure Legend Snippet: Schematic illustration of FG-4592 ameliorating TIF. During CKD, FG-4592 transcriptionally upregulates Furin expression, which subsequently cleaves iFGF23, leading to a reduction in iFGF23 levels and amelioration of TIF
Techniques Used: Expressing
Related Articles
Concentration Assay:Article Title: The Roxadustat (FG-4592) ameliorates tubulointerstitial fibrosis by promoting intact FGF23 cleavage Article Snippet: The Scr, BUN, and hemoglobin levels were quantified using the commercial kits (Jiancheng, Nanjing, China). .. The iFGF23 concentration was examined on plasma collected from rats under fed conditions using ELISA assays specific for rat iFGF23 (Quidel, cat#: 60–6800). iFGF23 and cFGF23 concentration were examined on plasma collected from rats under fed conditions using ELISA assays specific for rat total FGF23 (Quidel, cat#: 60–6300). iFGF23 and cFGF23 concentration were examined on plasma collected from CKD patients under fed conditions using ELISA assays specific for Clinical Proteomics:Article Title: The Roxadustat (FG-4592) ameliorates tubulointerstitial fibrosis by promoting intact FGF23 cleavage Article Snippet: The Scr, BUN, and hemoglobin levels were quantified using the commercial kits (Jiancheng, Nanjing, China). .. The iFGF23 concentration was examined on plasma collected from rats under fed conditions using ELISA assays specific for rat iFGF23 (Quidel, cat#: 60–6800). iFGF23 and cFGF23 concentration were examined on plasma collected from rats under fed conditions using ELISA assays specific for rat total FGF23 (Quidel, cat#: 60–6300). iFGF23 and cFGF23 concentration were examined on plasma collected from CKD patients under fed conditions using ELISA assays specific for Enzyme-linked Immunosorbent Assay:Article Title: The Roxadustat (FG-4592) ameliorates tubulointerstitial fibrosis by promoting intact FGF23 cleavage Article Snippet: The Scr, BUN, and hemoglobin levels were quantified using the commercial kits (Jiancheng, Nanjing, China). .. The iFGF23 concentration was examined on plasma collected from rats under fed conditions using ELISA assays specific for rat iFGF23 (Quidel, cat#: 60–6800). iFGF23 and cFGF23 concentration were examined on plasma collected from rats under fed conditions using ELISA assays specific for rat total FGF23 (Quidel, cat#: 60–6300). iFGF23 and cFGF23 concentration were examined on plasma collected from CKD patients under fed conditions using ELISA assays specific for |
